茶饮料中茶多酚含量测定方法的研究

摘要：

【目的】建立一种福林酚衍生比色法测定茶饮料中茶多酚含量的分析方法。【方法】采用单因素试验确定衍生的最佳条件，在此条件下绘制没食子酸标准系列溶液吸光度与浓度的标准曲线，并测定实际样品，同时与国标方法测得的结果进行比对。【结果】确定了在10mL溶液体系中，对标准系列溶液的最佳衍生条件为：福林酚用量1.0mol，碳酸钠用量1.5mL，可在自然光线下进行，反应时长60min。所得标准曲线为y=0.100876x+0.015891，R2=0.9989，线性范围100～2000 mg/kg，方法回收率为106.9%，相对标准偏差为0.6%(n=6)。【结论】与现行国标方法相比，该法适用浓度范围更广，基质干扰和仪器系统误差更小，准确度和精密度也更高，可为国标方法的改进提供参考。

Abstract:

[Objective] The research aims to establish a Folin-Phenol derivative colorimetric analytical method for  determining the content of tea polyphenols in tea beverage. [Method] The optimal conditions for derivatization are determined  by one-factor experiments. Under those conditions, the standard curve of gallic acid is plotted by measuring absorbance  and concentration of the standard series solution, and then the practical samples are measured. The results are compared  with those measured by the national standard method. [Result] The optimal derivatization conditions for the standard series  solution in a 10 mL system are determined as follows: Folin-phenol reagent usage of 1.0 mol, sodium carbonate usage of 1.5  mL，and the reaction can be carried out under natural light for 60 min. The standard curve is y=0.100876x + 0.015891, R2  =0.9989, with a linear range of 100~2000 mg/kg, a method recovery rate of 106.9%, and a relative standard deviation of 0.6%  (n=6). [Conclusion] Compared to the national standard method, the method has a wider applicable range of concentration,  smaller matrix interference and instrumental system errors, and higher accuracy and precision, which can provide reference  for improving the national standard method. 

引用格式：高树财,闫格,胡桂芳,等.茶饮料中茶多酚含量测定方法的研究[J].标准科学,2025(1):99-103.

基金项目：本文系河南省科学院食品安全与营养健康创新团队项目（项目编号：20230104）研究成果。 

作者简介：高树财，硕士，助理工程师，研究方向为食品安全检测技术研究。 高火亮，通信作者，本科，工程师，研究方向为食品安全检测技术研究。